{"id":2770,"date":"2019-06-17T11:32:31","date_gmt":"2019-06-17T09:32:31","guid":{"rendered":"https:\/\/int-ecole-org.flywheelsites.com\/?page_id=2770"},"modified":"2026-03-09T18:11:55","modified_gmt":"2026-03-09T17:11:55","slug":"plateforme-imagerie-3d","status":"publish","type":"page","link":"https:\/\/www.supbiotech.fr\/recherche-innovation-biotechnologie\/plateforme-imagerie-3d\/","title":{"rendered":"Plateforme Imagerie 3DBioDive CellTechs\/SupBiotech\/CEA"},"content":{"rendered":"\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"246\" height=\"188\" src=\"https:\/\/www.supbiotech.fr\/recherche-innovation-biotechnologie\/wp-content\/uploads\/2026\/03\/Image3.jpg\" alt=\"\" class=\"wp-image-5729\" style=\"width:294px;height:auto\"\/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<p>Le laboratoire <strong>CellTechs<\/strong>, en partenariat avec le <strong>CEA-SEPIA<\/strong>, s\u2019est dot\u00e9 en 2018 d\u2019un <strong>microscope \u00e0 feuillet de lumi\u00e8re (Light-Sheet Ultramicroscope II)<\/strong>, et en 2023 d&rsquo;un microscope Thunder,  instruments de pointe permettant l\u2019imagerie tridimensionnelle d\u2019\u00e9chantillons biologiques complexes tels que les organo\u00efdes c\u00e9r\u00e9braux. Ces microscopes permettent de visualiser des structures en 3D  tout en minimisant la phototoxicit\u00e9 et le photoblanchiment. Les appareils sont ouvert \u00e0 la communaut\u00e9 scientifique : chercheurs, partenaires acad\u00e9miques ou industriels, ainsi qu\u2019aux <strong>\u00e9tudiants de SupBiotech<\/strong> dans le cadre des travaux pratiques et projets de recherche.<\/p>\n\n\n\n<p>Pour toute demande d\u2019information ou de r\u00e9servation, contactez : <strong>elise.delage@supbiotech.fr<\/strong><\/p>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p class=\"has-large-font-size\"><strong>Microscopie \u00e0 feuillet de lumi\u00e8re (LSFM)<\/strong><\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<p><\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"923\" height=\"1024\" src=\"https:\/\/www.supbiotech.fr\/recherche-innovation-biotechnologie\/wp-content\/uploads\/2025\/11\/maxime-micro-lsfm-923x1024.jpg\" alt=\"\" class=\"wp-image-5393\" srcset=\"https:\/\/www.supbiotech.fr\/recherche-innovation-biotechnologie\/wp-content\/uploads\/2025\/11\/maxime-micro-lsfm-923x1024.jpg 923w, https:\/\/www.supbiotech.fr\/recherche-innovation-biotechnologie\/wp-content\/uploads\/2025\/11\/maxime-micro-lsfm-270x300.jpg 270w, https:\/\/www.supbiotech.fr\/recherche-innovation-biotechnologie\/wp-content\/uploads\/2025\/11\/maxime-micro-lsfm-768x852.jpg 768w, https:\/\/www.supbiotech.fr\/recherche-innovation-biotechnologie\/wp-content\/uploads\/2025\/11\/maxime-micro-lsfm-1384x1536.jpg 1384w, https:\/\/www.supbiotech.fr\/recherche-innovation-biotechnologie\/wp-content\/uploads\/2025\/11\/maxime-micro-lsfm-1846x2048.jpg 1846w\" sizes=\"auto, (max-width: 923px) 100vw, 923px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<p class=\"has-small-font-size\">La <strong>microscopie \u00e0 feuillet de lumi\u00e8re<\/strong> (Light Sheet Fluorescence Microscopy \u2013 LSFM) utilise un fin plan de lumi\u00e8re pour illuminer l\u2019\u00e9chantillon, couche par couche, tandis qu\u2019un objectif perpendiculaire capture les images.<br>Cette technique permet de <strong>scanner rapidement des volumes entiers en 3D<\/strong> tout en limitant l\u2019exposition lumineuse, ce qui la rend particuli\u00e8rement adapt\u00e9e \u00e0 l\u2019imagerie d\u2019\u00e9chantillons vivants, d\u2019organo\u00efdes ou de tissus \u00e9pais.<br>Elle offre ainsi un compromis unique entre vitesse, r\u00e9solution et pr\u00e9servation de la viabilit\u00e9 cellulaire.<br><br>Cr\u00e9dits : <em><strong>Microscope \u00e0 Feuillet de Lumi\u00e8re (Light-Sheet, Miltenyi).<\/strong> Partenariat SupBiotech\/CEA<\/em><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<p class=\"has-large-font-size\"><strong>Microscopie Thunder (Leica)<\/strong><\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/www.supbiotech.fr\/recherche-innovation-biotechnologie\/wp-content\/uploads\/2025\/11\/thomas-1024x768.jpeg\" alt=\"\" class=\"wp-image-5394\" srcset=\"https:\/\/www.supbiotech.fr\/recherche-innovation-biotechnologie\/wp-content\/uploads\/2025\/11\/thomas-1024x768.jpeg 1024w, https:\/\/www.supbiotech.fr\/recherche-innovation-biotechnologie\/wp-content\/uploads\/2025\/11\/thomas-300x225.jpeg 300w, https:\/\/www.supbiotech.fr\/recherche-innovation-biotechnologie\/wp-content\/uploads\/2025\/11\/thomas-768x576.jpeg 768w, https:\/\/www.supbiotech.fr\/recherche-innovation-biotechnologie\/wp-content\/uploads\/2025\/11\/thomas-1536x1152.jpeg 1536w, https:\/\/www.supbiotech.fr\/recherche-innovation-biotechnologie\/wp-content\/uploads\/2025\/11\/thomas-2048x1536.jpeg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<p>La <strong>microscopie Thunder<\/strong> (Leica) combine la microscopie \u00e0 champ large classique avec un algorithme de <strong>computational clearing<\/strong>, qui \u00e9limine la lumi\u00e8re diffus\u00e9e hors du plan focal.<br>Elle permet d\u2019obtenir des images nettes et contrast\u00e9es en temps r\u00e9el, sans n\u00e9cessiter de d\u00e9tection confocale. Cette technologie est id\u00e9ale pour l\u2019observation d\u2019\u00e9chantillons \u00e9pais, de tissus biologiques ou d\u2019organo\u00efdes, et constitue un outil compl\u00e9mentaire \u00e0 la microscopie Light Sheet pour la <strong>visualisation rapide et pr\u00e9cise en 3D<\/strong>.<\/p>\n\n\n\n<p class=\"has-small-font-size\">Cr\u00e9dits : <em><strong>Microscope Thunder (Leica).<\/strong> Partenariat SupBiotech\/CEA<\/em>. Remerciements \u00e0 DIM CBRAINS (R\u00e9gion Ile-de-France) pour la participation \u00e0 l&rsquo;acquisition du mat\u00e9riel.<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"1115\" height=\"971\" src=\"https:\/\/www.supbiotech.fr\/recherche-innovation-biotechnologie\/wp-content\/uploads\/2026\/03\/logo-cbrains-carre.png\" alt=\"\" class=\"wp-image-5730\" srcset=\"https:\/\/www.supbiotech.fr\/recherche-innovation-biotechnologie\/wp-content\/uploads\/2026\/03\/logo-cbrains-carre.png 1115w, https:\/\/www.supbiotech.fr\/recherche-innovation-biotechnologie\/wp-content\/uploads\/2026\/03\/logo-cbrains-carre-300x261.png 300w, https:\/\/www.supbiotech.fr\/recherche-innovation-biotechnologie\/wp-content\/uploads\/2026\/03\/logo-cbrains-carre-1024x892.png 1024w, https:\/\/www.supbiotech.fr\/recherche-innovation-biotechnologie\/wp-content\/uploads\/2026\/03\/logo-cbrains-carre-768x669.png 768w\" sizes=\"auto, (max-width: 1115px) 100vw, 1115px\" \/><\/figure>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Le laboratoire CellTechs, en partenariat avec le CEA-SEPIA, s\u2019est dot\u00e9 en 2018 d\u2019un microscope \u00e0 feuillet de lumi\u00e8re (Light-Sheet Ultramicroscope II), et en 2023 d&rsquo;un microscope Thunder, instruments de pointe permettant l\u2019imagerie tridimensionnelle d\u2019\u00e9chantillons biologiques complexes tels que les organo\u00efdes c\u00e9r\u00e9braux. Ces microscopes permettent de visualiser des structures en 3D tout en minimisant la phototoxicit\u00e9 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":1839,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"banner-fil-dariane","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-2770","page","type-page","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.2 (Yoast SEO v27.2) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Plateforme Imagerie 3DBioDive CellTechs\/SupBiotech\/CEA - La recherche \u00e0 SupBiotech<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.supbiotech.fr\/recherche-innovation-biotechnologie\/plateforme-imagerie-3d\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Plateforme Imagerie 3DBioDive CellTechs\/SupBiotech\/CEA\" \/>\n<meta property=\"og:description\" content=\"Le laboratoire CellTechs, en partenariat avec le CEA-SEPIA, s\u2019est dot\u00e9 en 2018 d\u2019un microscope \u00e0 feuillet de lumi\u00e8re (Light-Sheet Ultramicroscope II), et en 2023 d&rsquo;un microscope Thunder, instruments de pointe permettant l\u2019imagerie tridimensionnelle d\u2019\u00e9chantillons biologiques complexes tels que les organo\u00efdes c\u00e9r\u00e9braux. 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